5-Bromo-4-chloro-3-indolyl-b-D-galactopyranoside, X-GAL CAS 7240-90-6

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Product Descriptions:

5-Bromo-4-chloro-3-indolyl-β-D- galactopyranoside, commonly known as X-GAL (CAS 7240-90-6), is a colorless to white crystalline substance with the ability to produce a blue precipitate when cleaved by β-galactosidase. It is widely used as a chromogenic substrate in molecular biology for the detection of gene expression and in various techniques, such as blue-white screening and recombinant DNA technology, for the identification and selection of transformed cells.

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Synonym BCIG, 5-Bromo-4-chloro-3-indolyl β-D-galactoside, X-Gal
CAS 7240-90-6
Molecular Formula C14H15BrClNO6
Molecular Weight 408.63 g/mol
Density N.A
Product Category Sugars & Carbohydrates | Enzyme Substraes | Biochemicals | Diagnostic Reagents
Appearance White to Almost white powder to crystal
Purity Min 97%
HS Code 29389090
Specific Rotation -66° (C=1,DMF)
Store Under Inert Gas Store under inert gas
Condition to Avoid Light Sensitive, Air Sensitive, Heat Sensitive
PubChem Substance ID 87560918
MDL Number MFCD00005666
Physical State (20 deg.C) Solid
    • Gene Expression Analysis: Used in molecular biology as a chromogenic substrate for the detection of β-galactosidase activity, enabling the visualization and quantification of gene expression in various cell-based assays.
    • Recombinant DNA Technology: Employed as a marker in recombinant DNA technology to distinguish between recombinant and non-recombinant plasmids, facilitating the identification and selection of transformed cells.
    • Blue-White Screening: Applied in blue-white screening assays to distinguish between colonies containing plasmids with inserts and those without, aiding in the efficient identification and isolation of recombinant DNA clones.
    • Cloning Experiments: Utilized in cloning experiments and molecular genetics research to assess the efficiency of DNA fragment insertion into vectors, supporting the study of gene function and regulation in various biological systems.